内科 - 《内科》编辑部

目的分析环状RNA（circRNA）-ASAP2在结直肠癌诊断和淋巴结转移风险判断中的价值。方法选取2020年1月至2022年5月于右江民族医学院附属医院接受治疗的100例结直肠癌患者为结直肠癌组，另选100例体检健康者为对照组。比较血清、癌组织和癌旁组织中circRNA-ASAP2相对表达水平，以及不同特征的结直肠癌患者血清、癌组织circRNA-ASAP2相对表达水平，并应用受试者操作特征（ROC）曲线分析血清circRNA-ASAP2诊断结直肠癌和预测淋巴结转移的价值。结果结直肠癌组患者血清circRNA-ASAP2相对表达水平高于对照组，癌组织circRNA-ASAP2相对表达水平高于癌旁组织（均P<0.05）。发生淋巴结转移、临床分期为Ⅲ期～Ⅳ期的结直肠癌患者血清、癌组织circRNA-ASAP2相对表达水平均较高（均P<0.05）；不同性别、年龄、肿瘤长径、肿瘤类型、分化程度的结直肠癌患者血清、癌组织circRNA-ASAP2相对表达水平差异均无统计学意义（均P>0.05）。血清circRNA-ASAP2诊断结直肠癌的ROC曲线下面积为0.911（95%CI：0.848～0.975），敏感性为95.60%，特异性为72.2%；血清circRNA-ASAP2预测结直肠癌淋巴结转移的ROC曲线下面积为0.813（95%CI：0.696～0.930），敏感性为75.00%，特异性为90.78%。结论 circRNA-ASAP2在结直肠癌患者癌组织和血清中的表达上调，circRNA-ASAP2在结直肠癌的诊断和淋巴结转移风险判断中有一定的价值。

Objective To analyze the value of circular RNA (circRNA)-ASAP2 in diagnosing colorectal cancer and assessing the risk of lymph node metastasis. Methods A total of 100 colorectal cancer patients who were treated in the Affiliated Hospital of Youjiang Medical University for Nationalities from January 2020 to May 2022 were selected as the colorectal cancer group, and another 100 healthy individuals who underwent physical examinations were selected as the control group. Relative expression levels of circRNA-ASAP2 in the serum, cancer tissue, and pericarcinous tissue were compared, and the levels in the serum and cancer tissue between colorectal cancer patients with different characteristics were compared. The receiver operating characteristic (ROC) curve was used to analyze the value of serum circRNA-ASAP2 in diagnosing colorectal cancer and predicting lymph node metastasis. Results The serum relative expression level of circRNA-ASAP2 in the colorectal cancer group was higher than that in the control group, and the cancer tissue relative expression level of circRNA-ASAP2 was higher than the pericarcinous tissue level (all P<0.05). The serum and cancer tissue relative expression levels of circRNA-ASAP2 were higher in colorectal cancer patients with lymph node metastasis and with Ⅲ-Ⅳ clinical stage (all P<0.05). There was no statistically significant difference in the serum or cancer tissue relative expression level of circRNA-ASAP2 between colorectal cancer patients with different genders, ages, tumor lengths, tumor types, or differentiation degrees (all P>0.05). The area under the ROC curve of serum circRNA-ASAP2 in diagnosing colorectal cancer was 0.911 (95%CI: 0.848-0.975), with a sensitivity of 95.60% and a specificity of 72.2%; the area under the ROC curve of serum circRNA-ASAP2 in predicting lymph node metastasis in colorectal cancer was 0.813 (95%CI: 0.696-0.930), with a sensitivity of 75.00% and a specificity of 90.78%. Conclusion Colorectal cancer patients have up-regulated circRNA-ASAP2 expression in cancer tissues and serum, and circRNA-ASAP2 has a certain value in the diagnosis of colorectal cancer and the assessment of lymph node metastasis risk.

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