【Abstract】 Objective To investigate the value of levator hiatus area (LHA) measured by transperineal pelvic floor dynamic three-dimensional ultrasound (3DUS) in the diagnosis of pelvic organ prolapse (POP). Methods A total of 42 POP patients (POP group) and 42 healthy women without POP (non-POP group) were enrolled as study subjects. The LHA measured by transperineal pelvic floor dynamic 3DUS on the maximum Valsalva maneuver was compared between the two groups, and the receiver operating characteristic (ROC) curve was used to evaluate the value of LHA measured by transperineal pelvic floor dynamic 3DUS in the diagnosis of POP. Results LHA on the maximum Valsalva maneuver, measured by transperineal pelvic floor dynamic 3DUS, was larger in the POP group than in the non-POP group (P<0.05), and its area under the ROC curve in the diagnosis of POP was 0.920 (95% CI: 0.858-0.982, P<0.001), with the optimal cut-off value, specificity, sensitivity, and Youden index of 17.515 cm2, 0.833, 0.881, 0.714,respectively. Conclusion LHA measured by transperineal pelvic floor dynamic 3DUS on the maximum Valsalva maneuver has a good diagnostic value for POP, and the sensitivity and specificity of the diagnosis are high.