【Abstract】 Objective To investigate the relocation stress of the main caregivers of children transferred from the pediatric intensive care units (PICU) and to analyze its influencing factors. Methods A total of 150 main caregivers of children transferred from the PICU were selected as the study subjects, and the general data of the children and their main caregivers were collected. The Family Relocation Stress Scale for Patient Transferred From Intensive Care Units(FRSS) was used to assess the relocation stress level of the main caregivers, and the univariate analysis and the multivariate linear regression model were used to analyze the influencing factors for the relocation stress level of the main caregivers of children transferred from PICU. Results The total score of FRSS of the main caregivers of children transferred from PICU was (38.92±8.93). The univariate analysis results showed that there were statistically significant differences in the total scores of FRSS between the main caregivers of children transferred from PICU with different genders, education levels, monthly family incomes, working status, places of residence, nursing experiences, understanding of the disease, and PICU treatment time of the children (all P<0.05). The results of multivariate linear regression analysis showed that the gender and the understanding of the disease of main caregivers, as well as the PICU treatment time of the children, were the influencing factors for the relocation stress level of main caregivers of children transferred from PICU; female main caregivers had a higher relocation stress level than males, main caregivers who had a lower understanding of disease or whose children stayed in the PICU for a longer time had a higher relocation stress level (all P<0.05). Conclusion The relocation stress of the main caregivers of children transferred from PICU is at a low to medium level, which is mainly influenced by the gender and the understanding of the disease of main caregivers, as well as the PICU treatment time of the children.